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. 2021 Oct 23;9(11):1236. doi: 10.3390/vaccines9111236

Figure 6.

Figure 6

Determination of homing of mucosal T cells. Mice were inoculated with PBS, CD40L/HIV VLPs, and C1q conjugated CD40L/HIV VLPs at sublingual site for 7 days. Cervical lymph nodes were extracted and processed with standard frozen section protocol. The samples were stained with AF488 labeled anti-CD3e antibody and PE labeled anti-Integrin β7 or CCR9 antibodies. Lymphocytes from mouse cervical lymph node sections were co-stained with (A) CD3e and CCR9 antibodies or with (B) CD3e and integrin β7 antibodies. Scale bar, 25 μm. (C,D) The quantifications for immunofluorescence staining were determined by counting the positive cell numbers in each sample. Five random fields were selected and used to count numbers for each sample. Error bars represent means ± SEM. Data were analyzed by using Two-way ANOVA and unpaired Student’s t test. * indicates p < 0.05 compared to PBS. ## indicates p < 0.01 compared to CD40L/HIV VLPs.