LOE and losartan treatments prevent vascular generation of reactive oxygen species in aortic sections derived from apoE−/− mice. The aortic sections were exposed to dihydroethidine (DHE, 2.5 µM), a redox-sensitive fluorescent dye, for 30 min. Subsequently, ethidium fluorescence was evaluated via confocal microscopy. (a) Representative images show H&E staining (top), DHE staining in red (middle), and merged images (bottom). (b) Corresponding cumulative data. The scale bar represents 100 µm. The results are shown as mean ± SEM (n = 4–6). * p < 0.05 versus the C57BL/6 group and #
p < 0.05 versus the apoE−/− vehicle group.