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. 2021 Nov 18;10(11):2493. doi: 10.3390/plants10112493

Figure 3.

Figure 3

LOE and losartan treatments inhibit NADPH oxidase subunits p22phox and p47phox in aortic sections obtained from apoE−/− mice. The expression of the NADPH oxidase subunits p22phox and p47phox was determined by confocal microscopy using a purified polyclonal antibody and a fluorescence-tagged secondary antibody. Nuclei were stained with DAPI (blue). (a) Representative images show H&E staining (top), p22phox and p47phox staining in red (middle), and merged fluorescence staining and DAPI (bottom). (b) Corresponding cumulative data. The scale bar represents 100 µm. The results are shown as mean ± SEM (n = 4–6). * p < 0.05 versus the C57BL/6 group and # p < 0.05 versus the apoE−/− vehicle group.