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. 2021 Nov 4;10(11):1433. doi: 10.3390/pathogens10111433

Table 4.

Description of the primers used in this study for gene amplification as well as sequencing.

Target
Gene
Primer
Name
Sequence (5′–3′) Tm (°C) PCR Amplicon
Length (bp)
Sequencing References
18S rRNA CRYPTOF AACCTGGTTGATCCTGCCAGTAGTCAT 63 × 1728 × [33]
CRYPTOR TGATCCTTCTGCAGGTTCACCTA × ×
BAB-GF2 GTCTTGTAATTGGAATGATGG 61 × 560 × [11]
BAB-GR2 CCAAAGACTTTGATTTCTCT × ×
ama-1 ama1-S1 TGACTGCCATATCGACGAAG 61 × ≈2000 × this study
ama1-R3 CTCTAGTGAATTACGATAGC × × [50]
ama1-As1 GGCGGATATTCGGTTGAGG × this study
ama1-S2 CATGGCCAAGTTTGACCTTG × this study
ama1-As2 CTGCGTCACGCGTGAATTC × this study
ama1-S3 CTCCTGTGTATGGAGCCGA × this study
ama1-As3 GTGAAAGCGCGGTTGTGAC × this study
ama1-S4 AGCAGTTGGATCGCCTCTC × this study
rap-1a rap1-fw AATGTCCTACTGGGAAACGC 58 × ≈1300 × this study
rap1-rev GCGGAGTCCATGCCTGTACC × × this study
rap-1a1 (5′) rap1-fw see above 58 × 1146 ×
rap1-a1-rev GCTTAGTAGCATGCATCTTC × × this study
rap-1a1 (3′) rap1-a1-fw GGACTCCGAGAAAAAGGATG 58 × 261 × this study
rap1-rev see above × ×
rap-1a2 (5′) rap1-fw see above 58 × 1123 ×
rap1-a2-rev TGGAACAACTTCTTCATAGG × × this study
rap-1a2 (3′) rap1-a2-fw GGGCTTCTGGAAAAAGAAGG 58 × 228 × this study
rap1-rev see above × ×