Table 4.
Target Gene |
Primer Name |
Sequence (5′–3′) | Tm (°C) | PCR | Amplicon Length (bp) |
Sequencing | References |
---|---|---|---|---|---|---|---|
18S rRNA | CRYPTOF | AACCTGGTTGATCCTGCCAGTAGTCAT | 63 | × | 1728 | × | [33] |
CRYPTOR | TGATCCTTCTGCAGGTTCACCTA | × | × | ||||
BAB-GF2 | GTCTTGTAATTGGAATGATGG | 61 | × | 560 | × | [11] | |
BAB-GR2 | CCAAAGACTTTGATTTCTCT | × | × | ||||
ama-1 | ama1-S1 | TGACTGCCATATCGACGAAG | 61 | × | ≈2000 | × | this study |
ama1-R3 | CTCTAGTGAATTACGATAGC | × | × | [50] | |||
ama1-As1 | GGCGGATATTCGGTTGAGG | × | this study | ||||
ama1-S2 | CATGGCCAAGTTTGACCTTG | × | this study | ||||
ama1-As2 | CTGCGTCACGCGTGAATTC | × | this study | ||||
ama1-S3 | CTCCTGTGTATGGAGCCGA | × | this study | ||||
ama1-As3 | GTGAAAGCGCGGTTGTGAC | × | this study | ||||
ama1-S4 | AGCAGTTGGATCGCCTCTC | × | this study | ||||
rap-1a | rap1-fw | AATGTCCTACTGGGAAACGC | 58 | × | ≈1300 | × | this study |
rap1-rev | GCGGAGTCCATGCCTGTACC | × | × | this study | |||
rap-1a1 (5′) | rap1-fw | see above | 58 | × | 1146 | × | |
rap1-a1-rev | GCTTAGTAGCATGCATCTTC | × | × | this study | |||
rap-1a1 (3′) | rap1-a1-fw | GGACTCCGAGAAAAAGGATG | 58 | × | 261 | × | this study |
rap1-rev | see above | × | × | ||||
rap-1a2 (5′) | rap1-fw | see above | 58 | × | 1123 | × | |
rap1-a2-rev | TGGAACAACTTCTTCATAGG | × | × | this study | |||
rap-1a2 (3′) | rap1-a2-fw | GGGCTTCTGGAAAAAGAAGG | 58 | × | 228 | × | this study |
rap1-rev | see above | × | × |