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. 2021 Nov 22;22(22):12597. doi: 10.3390/ijms222212597

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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The roles of AA metabolism during SGIV replication. (A) The cytotoxicity of 2-TEDC or Ibuprofen on GS cells was determined using MTT assay. (B) The severity of CPE induced by SGIV infection at 24 h p.i. in 2-TEDC or Ibuprofen-treated cells. (C) Western blotting analysis of viral MCP during SGIV infection in 2-TEDC or Ibuprofen-treated cells. (D,E) The viral gene transcription levels of SGIV, MCP, and VP19 were detected in infected cells after treatment with 2-TEDC or Ibuprofen by qPCR, respectively. (F,G) The effects of 2-TEDC or Ibuprofen on virus titers during SGIV infection were detected by virus plaque assay. Data are expressed as means ± SD. The asterisks (*) indicated p < 0.05.