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. 2021 Oct 22;19(11):599. doi: 10.3390/md19110599

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Act V exerts a parallel apoptotic effect to the PI3K inhibitor LY294002. (A) Flow cytometry detected the apoptotic cell proportion after HCT-116 cells were inoculated and treated with Act V, LY294002, or a dual treatment. (B) The proportion of apoptotic cells was statistically recorded. (C) The PI3K expression was investigated using Act V and the PI3K inhibitor LY294002: HCT-116 cells treated with dual agents expressed a dramatically low level of PI3K protein compared with the single agent alone. * p < 0.05, *** p < 0.001.