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. 2000 Sep;20(18):6721–6730. doi: 10.1128/mcb.20.18.6721-6730.2000

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Copyright © 2000, American Society for Microbiology

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FIG. 6 — HCF_SAS1 function is conserved between HCF-1 and HCF-2. T7-tagged HCF-1_C1758–2035 was coexpressed in transfected 293T cells with HA-tagged fragments (fused to GAL4 residues 1 to 94) corresponding to residues 341 to 394 of HCF-2 (lane 1), 348 to 450 of HCF-1 (lane 2), and 382 to 450 of HCF-1 (lane 3). HCF-1_SAS1N and HCF-1_SAS1C association was assayed by coimmunoprecipitation (IP) with the αHA antibody followed by immunoblotting with the αT7 antibody (upper panel). Protein expression was confirmed by immunoblotting extracts directly with the αT7 (center panel; HCF-1_C polypeptides) or αHA (lower panel; HCF-1_C polypeptides) antibody.