Figure 2.

Immunogenicity examination of CuMV_TT-FP and CuMV_TT-DF vaccines in mice. (A): Immunization regimen: BALB/c mice were subcutaneously injected with 100 ug CuMV_TT-FP or CuMV_TT-DF vaccine at d0 and d21. Serum samples were collected every week until d49 (n = 5). (B–D): IgG antibody titers of immunized mice against RBD (B), S2 protein (C) and spike protein (D). Unpaired t-test analysis was performed in GraphPad Prism 7. α = 0.05 and statistical significance were displayed as p ≤ 0.05 (*), p ≤ 0.005 (***), p ≤ 0.001 (****).