Avidity indexes of IgG antibodies in mice before and after booster immunization of CuMVTT-FP and CuMVTT-DF (right panel, indicated as red) measured by avidity ELISA (n = 5). (A,B): Avidity indexes of IgG antibodies for RBD from CuMVTT-FP (A) or CuMVTT-DF (B) immunized mice; (C,D): Avidity indexes for spike S2 protein of CuMVTT-FP (C) or CuMVTT-DF (D) elicited IgG antibodies; (E,F): Avidity indexes for spike S2 protein of CuMVTT-FP (E) or CuMVTT-DF (F) stimulated IgG antibodies. Paired t-test in GraphPad Prism 7 was used for statistical analysis. α = 0.05 and statistical significance were displayed as p ≤ 0.05 (*), p ≤ 0.01 (**).