FIG. 8.

BAP31 associates with constituents of the actomyosin complex. Extracts of HepG2 cells, either lacking or expressing BAP31-Flag, were subjected to immunoprecipitation with the anti-Flag antibody (α Flag) (31), and the precipitates were analyzed by SDS-PAGE and silver staining. The positions of BAP31-Flag and BAP31 were determined separately by immunoblotting with anti-BAP31 (not shown). Molecular mass markers and the light (LC) and heavy (HC) chains of IgG are also indicated. The bands labeled non-muscle myosin II HC B and γ-actin were identified by tandem mass spectrometry (see Results and Discussion).