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. 2021 Nov 10;7(11):947. doi: 10.3390/jof7110947

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Transcriptomic analysis to identify DEGs in ∆UvCCHC4-1 vs. HWD-2. (A) Volcano plot of DEGs in ∆UvCCHC4-1 vs. HWD-2. (B) Heatmap of FRKM-normalized transcript levels of the 810 DEGs in each pair-wise comparison. (C) GO enrichment analysis of the DEGs. MF: Molecular function; BP: Biological process; CC: Cellular component. (D) KEGG enrichment analysis of the DEGs. (E) RT-qPCR to validate the DEGs. The relative transcript abundance of each gene from ΔUvCCHC4-1 was normalized by comparison with the gene from HWD-2 (relative transcript level = 1). The asterisks represent significant differences between ΔUvCCHC4-1 and HWD-2 at p = 0.05.