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. 2021 Nov 19;22(22):12483. doi: 10.3390/ijms222212483

Figure 2.

Figure 2

The changes in DNMT1 localization and level in the neurons of ischemic penumbra at 4 and 24 h after photothrombotic stroke (PTS) in the rat cerebral cortex. The significant difference in localization and level of DNMT1 at 4 and 24 h after PTS (PTS4 and PTS24, respectively) is shown compared to the contralateral cortex of the same animals (CL4 and CL24), or the cerebral cortex of sham-operated rats (SO). (a) Immunofluorescence of DNMT1 (green), neuronal marker NeuN (red), nuclear chromatin marker Hoechst 33342 (blue), and image overlay. The scale bar is 50 μm. (b) Percent changes (ΔISO) of DNMT1 levels in the penumbra (PTS4 or PTS24) and CL4 or CL24 compared to SO at PTS4 or PTS24. (c) Percent changes (ΔICL) of DNMT1 levels in the penumbra at PTS4 or PTS24 compared to CL4 and CL24. (d) Coefficient M1 of DNMT1 co-localization with the neuronal marker (NeuN) in different control and experimental groups. (e) Immunofluorescence of DNMT1 in cell nuclei. (f) Percentage of cells expressing DNMT1 per 100 cells stained with Hoechst 33342 * p < 0.05 compared to SO, # p < 0.05 compared to CL4 and CL24 of the same animals. One Way ANOVA; M ± SEM; n = 7.