Figure 13.

Apoptosis in the peri-infarct (P) area induced by photothrombotic stroke (PTS) in the mouse cerebral cortex. (a,b) Typical images of cortical regions stained with TUNEL (red fluorescence of apoptotic cells) at 4 days (a) and 7 days (b) after PTS. P-peri-infarct area, I-infarct area, N-normal tissue area. Control: sham-operated animals (SO). Experimental groups: The cerebral cortex of mice injected by various inhibitors. Inhibitors: 5-aza-2′-deoxycytidine (5-aza-D), a selective DNMT1 inhibitor; A-366 and BIX01294, a nonspecific inhibitor of Suv39H1 and G9a. Scale bar 200 μm, and the immunofluorescence of TUNEL in the peri-infarct (P) area at bigger magnification (Scale bar 50 μm) (c) Changes in the apoptotic index (AI, %) in the mice of experimental groups at 4 and 7 days after PTS introduction of various inhibitors. (d) The effects of inhibitors of DNMT1 and methyltransferases Suv39H1 and G9a on the infarction core volume in the mouse brain at 4 and 7 days after PTS. Mean values of the infarction core volume (mm³) in the control group (PTS without inhibitors) and in the experimental groups (administration of inhibitors). Scale bar 1 cm. One Way ANOVA; M ± SEM; n = 7–10. * p < 0.05 compared to the PTS in the absence of inhibitors.