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Treatment scheme and sample preparation. Enrolment started on day 1 before the initiation of hormone treatment. A serum sample was prepared prior to follicular puncture and follicular fluid collection. Oocytes and embryos were evaluated based on morphological characteristics, and embryos were cultured in time-lapse systems after fertilization for recording morphokinetic times. ECC; embryo cell cycle, s; synchronization within an ECC.
