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Conditions of PCR reactions of the different primer pairs. Tann = annealing temperature.
| Primer Combination | Amplicon Length | Tann/Elongation Time |
|---|---|---|
| Cox1_schist_5k/Cox1_schist_3k | 1244 bp | 50 °C/90 s |
| Tricho_tRNA_fw/Tricho_tRNA_rv_2 | 401–482 bp | 54 °C/60 s |
| Cox1_schist_5_trich/CO1560R_modif | 612 bp | 52 °C/60 s |
| Tricho_Fw2/Tricho_Rv2_2 | 491 bp | 49 °C/60 s |
| ZDOE-COI-fw/Tricho_rev_20 | 486 bp | 53 °C/60 s |
| LCO1490_ABOL_Moll_1/HCO2198_ABOL_Moll _1 | 704 bp | 50 °C/60 s |
