Depolarization of the fungal membrane of C. albicans 1408 cell fungal membrane was assessed using diSC(3) assay. The evaluation of the degree of cell membrane depolarization in the presence of CSA-13, AuR NPs@CSA-13, AuP NPs@CSA-13, AuS NPs@CSA-13 (A); CSA-44, AuR NPs@CSA-44, AuP NPs@CSA-44, AuS NPs@CSA-44 (B); and CSA-131, AuR NPs@CSA-131, AuP NPs@CSA-131, AuS NPs@CSA-131 (C) ranging from 1 to 10 μg/mL was evaluated by monitoring the enhancement of intensity of the fluorescence. The dashed line represents the effect obtained with the amphotericin B at a concentration of 1 μg/mL. Results show the mean ± SD, n = 3; *** indicates statistical significance at ≤0.001.