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. 2021 Nov 1;13(11):1827. doi: 10.3390/pharmaceutics13111827

Figure 5.

Figure 5

Nephrin and podocin levels in aspirin + Li-treated rats. Rats were fed regular food (control) or lithium-containing food [LLD-Li or STD-Li] for 42 days. Low-dose aspirin (1 mg/kg, ip) was given alone or together with lithium. On day 42 the rats were sacrificed, kidneys excised and homogenized, and nephrin (a) and podocin (b) levels determined by ELISA as described in Materials and Methods. Results are the means ± SEM of a single representative experiment out of two demonstrating a similar pattern with 8 rats per group in the depicted experiment. * p < 0.05 vs. Control, # p < 0.05 vs. LLD-Li, ^ p < 0.05 vs. STD-Li. (a) Nephrin—two-way ANOVA: ASA effect: F1,30 = 5.4, p = 0.027; Li effect: F2,30 = 3.35, p < 0.05; Interaction: F2,30 = 2.62, p = 0.089. Post hoc LSD: Control vs. STD Li, p ≤ 0.01; LLD Li vs. STD-Li, p ≤ 0.01; STD-Li + ASA vs. STD-Li, p = 0.003. (b) Podocin—two-way ANOVA: ASA effect: F1,30 = 6.018, p = 0.02; Li effect: F2,30 = 4.449, p = 0.02; Interaction: F2,30 = 8.121, p = 0.0015. Post hoc LSD: Control vs. STD Li, p ≤ 0.0004; LLD-Li + vs. STD-Li, p ≤ 0.0004; STD Li + ASA vs. STD Li p < 0.0001. Abbreviations: ASA—acetylsalicylic acid, LLD—low-low dose, Li—lithium, STD—standard dose.