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. 2021 Nov 14;11(11):3061. doi: 10.3390/nano11113061

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Cytotoxic effect of GO nanoparticles with or without NIR on Colon26 cells by FACS after staining of cells with Rh123. Representative dot-plots of FSC (forward side scattering) vs. SSC (side scattering) for Colon26 cells are presented. The population of viable cells was gated and the calculated percentage of live cells is given on the charts. (A) Colon26 viability after 24 h of cultivation with NPs. (B) Colon26 viability after 72 h of cultivation with NPs. Three repetitions of the experiment were carried out and results are the MEAN values of the calculated % of viable cells. Statistically significant differences are denoted with * p ˂ 0.05.