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. 2021 Nov 14;11(11):3061. doi: 10.3390/nano11113061

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Cytotoxic effect of GO nanoparticles with or without NIR on HT29 cells studied by FACS after staining with Rh123. Representative dot-plots of FSC (forward scattering) vs. SSC (side scattering) for HT29 cells are presented. The population of viable cells was selected and the percentage of live cells is given on the charts as a function of all cells detected. (A) HT29 viability after 24 h of cultivation with NPs. (B) HT29 viability after 72 h of cultivation with NPs. Values are MEAN from three repetitions of the experiment and statistically significant differences are denoted with * p ˂ 0.05.