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. 2021 Nov 14;11(11):3061. doi: 10.3390/nano11113061

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Mitotoxicity of GO nanoparticles and NIR by Rhodamin123 staining assessed by FACS observation. The mitochondrial activity was detected by FACS based on the incorporation of ∆Ψ-sensitive Rh123 fluorescent dye in viable cells. Histograms represent Rh123 fluorescence acquired by flow cytometer using FL1-H detector. The charts show the distribution of gated viable cells (FSC/SSC) according to the intensity of their Rh123 (FL1-H) fluorescence. (A) Colon26 cells after 24 h incubation with NPs. (B) Colon26 cells after 72 h of NPs treatment. (C) HT29 cell—24 h after incubation with NPs. (D) HT29 cells—72 h of treatment with NPs.