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. 2021 Nov 14;11(11):3061. doi: 10.3390/nano11113061

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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RT-qPCR for studying the effect of PEGylated GO nanoparticles with near-infrared laser irradiation on the activity of stress-responsive genes in colorectal carcinoma cells. (A) Fold change in the expression of the ATM gene. (B) Fold change in the expression of RAD51 (C) Fold change in the expression of TP53 (D) Fold change in the expression of BCC3 (PUMA) (E) Fold change in the expression of CDKN1A (p21) Three repetitions of the experiment were conducted and values represent the fold change in the expression of the studied genes as MEAN ± STDV. Red arrows denote significant alterations in the activity of certain genes after GO NPs treatment with and without NIR irradiation.

RT-qPCR for studying the effect of PEGylated GO nanoparticles with near-infrared laser irradiation on the activity of stress-responsive genes in colorectal carcinoma cells. (A) Fold change in the expression of the ATM gene. (B) Fold change in the expression of RAD51 (C) Fold change in the expression of TP53 (D) Fold change in the expression of BCC3 (PUMA) (E) Fold change in the expression of CDKN1A (p21) Three repetitions of the experiment were conducted and values represent the fold change in the expression of the studied genes as MEAN ± STDV. Red arrows denote significant alterations in the activity of certain genes after GO NPs treatment with and without NIR irradiation.