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Agarose gel (2%) electrophoresis image of RPA reactions using ybbW primers and gDNA E. coli TOP10 (1–2 ng) as a template. The initial reaction was divided into different fractions before the amplification. The original TwistDx assay was performed for shorter time (Lane 3–4: 10 min and Lane 5–6: 20 min) and smaller sample volumes (Lane 3–4: 25 μL and Lane 5–6: 12.5 μL) than those recommended by the manufacturer. The performance of RPA was considered satisfactory.
