Table 2.
Effects of the Hb:trehalose (TRE) ratio on the size, polydispersity (PDI), loading content (LC), entrapment efficiency (EE), and functionality of the resulting HbNPs-TRE 1.
| Sample ID | Hb (mg mL−1) |
Hb:TRE Ratio |
Size (nm) | PDI | LC (%) | EE (%) | Functional |
|---|---|---|---|---|---|---|---|
| HbNPs-4 | 50 | 1:0 | 277.7 | 0.082 | 9.0 | 30.1 | No |
| HbNPs-4 TRE1 | 50 | 50:1 | 291.6 | 0.110 | 8.4 | 21.0 | No |
| HbNPs-4 TRE2 | 50 | 20:1 | 267.6 | 0.070 | 5.4 | 18.7 | No |
| HbNPs-4 TRE3 | 50 | 10:1 | 285.8 | 0.087 | 5.7 | 19.3 | Semi |
| HbNPs-4 TRE4 | 50 | 5:1 | 335.2 | 0.224 | 4.5 | 17.1 | Yes |
| HbNPs-4 TRE5 | 50 | 2:1 | 337.0 | 0.230 | 2.5 | 11.1 | Yes |
| HbNPs-4 TRE6 | 50 | 1:1 | 330.6 | 0.136 | 2.2 | 8.7 | Yes |
| HbNPs-5 | 75 | 1:0 | 313.9 | 0.156 | 14.9 | 32.6 | No |
| HbNPs-5 TRE1 | 75 | 50:1 | 286.1 | 0.109 | 11.5 | 26.7 | No |
| HbNPs-5 TRE2 | 75 | 20:1 | 281.8 | 0.102 | 7.8 | 17.9 | Semi |
| HbNPs-5 TRE3 | 75 | 10:1 | 296.2 | 0.101 | 5.0 | 15.2 | Semi |
| HbNPs-5 TRE4 | 75 | 5:1 | 284.6 | 0.191 | 2.9 | 8.0 | Semi |
| HbNPs-6 | 100 | 1:0 | 346.7 | 0.144 | 20.6 | 33.8 | Semi |
| HbNPs-6 TRE1 | 100 | 50:1 | 301.1 | 0.120 | 17.6 | 22.4 | Yes |
| HbNPs-6 TRE2 | 100 | 20:1 | 320.8 | 0.137 | 13.7 | 17.3 | Yes |
| HbNPs-6 TRE3 | 100 | 10:1 | 346.0 | 0.213 | 14.3 | 21.2 | Yes |
| HbNPs-6 TRE4 | 100 | 5:1 | 372.2 | 0.140 | 15.0 | 23.2 | Yes |
1 The HbNPs were prepared using 12.5 mg mL−1 PLGA and 1% PVA.