Table 4.
Additivity index (%) of the seven MAbs.
| MAbs | CBSSRBD-S.1 | CBSSRBD-S.2 | CBSSRBD-S.3 | CBSSRBD-S.4 | CBSSRBD-S.6 | CBSSRBD-S.7 | CBSSRBD-S.8 |
|---|---|---|---|---|---|---|---|
| CBSSRBD-S.1 | −8.05 | 88.27 | 47.15 | 13.90 | 4.46 | −16.09 | 73.61 |
| CBSSRBD-S.2 | 34.93 | 62.73 | 56.18 | 56.32 | 75.06 | 100.53 | |
| CBSSRBD-S.3 | 18.83 | 61.04 | 57.09 | 59.86 | 75.09 | ||
| CBSSRBD-S.4 | −3.13 | 14.14 | −1.35 | 67.66 | |||
| CBSSRBD-S.6 | 1.83 | 2.59 | 56.16 | ||||
| CBSSRBD-S.7 | 0.34 | 78.90 | |||||
| CBSSRBD-S.8 | 23.80 |
Data represent the AI calculated from triplicate absorbance values at 492 nm. The coefficient of variation of the absorbance values was less than 20%. The AI data arranged on the diagonal and highlighted in bold type, correspond to the AIzero. The mean of the AIzero was 9.79% and the SD was 16.06%. The cut point calculated, at 95% confidence, was AI≥41% (see formula defined above, for a t0.05, 6df value of 1.943). If the two antibodies bind randomly at the same site the AI will be ˂41%. If the two antibodies bind independently at distinct sites AI will be ≥41%.