Skip to main content
. 2021 Oct 25;7(11):901. doi: 10.3390/jof7110901

Figure 6.

Figure 6

Upc2/Ecm22 regulation via promoter iron-regulatory elements. (A) Upc2/Ecm22-dependent activation of FET3 upon iron deficiency occurs through its promoter. Wild-type (WT, W303) and upc2Δecm22Δ cells expressing PFET3-lacZ were grown at 30 °C in SC-Ura medium without (+Fe) or with 100 μM BPS (−Fe) for 6 h, and β-galactosidase activity was determined. Significant differences are represented by different letters above bars (p-value < 0.05). (B) FeRE sites are required for Upc2/Ecm22-mediated induction of transcription. Wild-type (WT, W303) and upc2Δecm22Δ cells expressing PCYC1-FeRE–lacZ were grown and analyzed as indicated in panel A. Data show the average and SD of three biologically independent experiments as compared to wild-type (+Fe) samples. Letters above bars indicate significant differences (p-value < 0.05).