Figure 8.

The phenotypes of upc2Δecm22Δ mutants under iron deficiency are partially rescued by the AFT1-1^up allele. Wild-type (WT, W303) cells and the upc2Δecm22Δ mutant were transformed with either pRS416 (vector) or pRS416-AFT1-1^up-12HA (AFT1-1^up) plasmids. (A–C) AFT1-1^up allele rescues the activation of the iron regulon. Cells were grown as in Figure 6. RNA was extracted, and mRNA levels of FET3, FTR1, and ARN2 normalized to ACT1 were determined by RT-qPCR. Data indicate the average and SD of three biologically independent experiments (p-values < 0.05). (D) The growth defect of upc2Δecm22Δ mutants under iron scarcity is partially rescued by the AFT1-1^up allele. Growth in SC-Ura (+Fe) and SC-Ura with 300 μM Ferrozine (−Fe) was tested. A representative photograph of three biologically independent experiments is displayed.