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. 2021 Nov 21;9(11):2397. doi: 10.3390/microorganisms9112397

Figure 2.

Figure 2

Fractionation of culture supernatants of Bifidobacterium strains. (A) HPLC, HPLC-DAD chromatograms (detector λ254 nm) of CSs and MRS. CS of B. longum subsp. longum (NITE BP-02621, infant-type HRB) (red line) and CS of B. animalis subsp. lactis (DSM10140T, non-HRB) (green line) and MRS (black line). The fractions were collected every 1 min (from 3–22 min) and numbered from fractions 1–18. (B) HCA3 activity of the 18 fractions of MRS and CSs of NITE BP-02621 and DSM10140T. The values shown are the mean values (RLU) of triplicates ± SD. RLU, relative light units that were subtracted from the PBS baseline. (C) Concentration of 4-OH-PLA, LeuA, PLA, and ILA in 18 fractions. Fractions MRS (black); NITE BP-02621 (red); and DSM10140T (green). The values shown are the mean values (μM) of triplicates ± SD.