Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Nov 10;22(22):12163. doi: 10.3390/ijms222212163

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Pristane-induced arthritis upregulates BMP-4 expression in the hippocampus/dentate gyrus in male rats. (A) Representative immunofluorescent pictures show staining with anti-BMP-4 antibody in paraffin-embedded sections of brain tissue obtained from male and female DA rats: control (treated with saline); PIA onset (between 9th and 12th day after induction); PIA peak (between 16th and 20th day after induction); PIA remission (between 20th and 25th day after induction). (B) BMP-4 immunoreactivity in the hippocampus/dentate gyrus. The immunofluorescent signal quantification was performed using Cell F v3.1 software analysis on 12 regions of interest (3 slides/rat × six animals/group). Values are expressed as mean grey value ± SD (N = 24). One-way ANOVA followed by the post hoc Scheffé test: * p < 0.05, ** p < 0.01, and *** p < 0.001. Insert shows staining in a slide incubated without primary anti-BMP4 antibody (negative control). Scale bars indicate 50 μm.