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. Author manuscript; available in PMC: 2021 Nov 26.
Published in final edited form as: Nat Protoc. 2020 Jul 17;15(8):2589–2610. doi: 10.1038/s41596-020-0345-1

Figure 2 |.

Figure 2 |

MS/MS spectra of site-specific O-GalNAc glycopeptides. The glycopeptides are generated using the protocol. O-GalNAc glycosylation sites are at the N-termini of peptide sequences. Different glycan compositions are showed to illustrate the approach for defining site-specific O-GalNAc glycans. (a) MS/MS spectra of a glycopeptide with Hex(1)HexNAc(1). (b) MS/MS spectra of a short glycopeptide with a HexNAc. (c) MS/MS spectra of a long glycopeptide with Hex(1)HexNAc(1). (d) MS/MS spectra of a glycopeptide with Hex(2)HexNAc(2).