| Input Data | |
| Protein Database | your database in FASTA format |
| Enzyme Name | Trypsin (semi) |
| Max. Missed Cleavage | 2 |
| Min. Peptide length | 6 |
| Max. Peptide length | 50 |
| Tolerances | |
| Precursor Mass Tolerance | 10 ppm |
| Fragment Mass Tolerance | 0.06 Da |
| Dynamic Modifications | |
| Oxidation | +15.995 Da at methionine |
| Guanidinyl | +42.022 Da at lysine |
| HexNAc | +203.079 Da at serine and threonine |
| Hex(1)HexNAc(1) | +365.132 Da at serine and threonine |
| Static Modification | |
| Carbamidomethyl | +57.021 Da at cysteine |
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