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. Author manuscript; available in PMC: 2021 Nov 26.
Published in final edited form as: Cell Rep. 2021 Nov 16;37(7):110008. doi: 10.1016/j.celrep.2021.110008

Figure 5. Sterol-mediated phase separation is required to support CME.

Figure 5.

(A) Summary of physical properties and phase separation behavior of selected sterols. Topological Polar Surface Area (TPSA) computed surface sum over all polar atoms. Ordered lipid (Lo) domain (raft) stabilizing (+) or disrupting (−) sterols are indicated relative to cholesterol (++). Refer to Table S2 for additional details.

(B) Sterol profiles of AY9944-treated HEK293T cells after sterol loading (mean ± SD). n = 4 independent biological replicates from 2 MβCD-sterol preparations.

(C) Tfn uptake relative to LPDS controls (mean ± SD). One-way ANOVA (F(12, 51) = 11.23, p < 0.0001) and Dunnett’s test versus LPDS control (n = 5 biological replicates from 3 independent experiments, ~1,500 cells per replicate). (c), commercially available MβCD-Chol.

(D) Representative mid-plane confocal images after incubation with AF-555 conjugated Tfn. Scale bar, 20 μm.