Table 2.
Main effects ANOVA of laccase activity of Cryphonectria parasitica isolates infected with different Cryphonectria hypovirus 1 strains or uninfected controls, when grown on different growth media for 5 or 10 days. Statistically significant differences at p < 0.05 are denoted by an asterisk (*).
| SOLID MEDIA | LIQUID MEDIA | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| smLAC | icLAC | ecLAC | ||||||||
| Source | Df 1 | MS 2 | F 3 | p | MS | F | p | MS | F | p |
| DURATION a | 1 | 102.694 | 8.9929 | 0.003 * | 9.90002 | 14.3188 | 0.0002 * | 0.005404 | 0.04547 | 0.8314 |
| MEDIUM RICHNESS b | 1 | 1265.916 | 110.8561 | <0.0001 * | 10.95784 | 15.8487 | 0.0001 * | 0.350012 | 2.94490 | 0.0877 |
| FUNGUS c | 3 | 65.347 | 5.7224 | 0.0009 * | 2.96914 | 4.2944 | 0.006 * | 0.230932 | 1.94300 | 0.1240 |
| VIRUS d | 4 | 126.814 | 11.1051 | <0.0001 * | 3.54686 | 5.1300 | 0.0006 * | 1.322938 | 11.13082 | <0.0001 * |
| Error | 194 | 11.419 | 0.69140 | 0.118854 | ||||||
1 degrees of freedom, 2 mean square value, 3 F ratio; NOTE: The following effects of the factors were tested: a duration of cultivation (five or ten days), b nutritional value of the growth medium (potato dextrose agar (PDA) or broth (PDB) were considered rich, while malt extract (ME) or malt extract agar (MEA) were considered poor), c fungal isolate and d virus strain on laccase activities of fungi grown either on solid media (PDA or MEA) or liquid media (PDB or ME). On solid media, laccase activity was defined as the sum of all intracellular and majority of extracellular laccase and designated smLAC, while on liquid media intracellular (icLAC) and extracellular (ecLAC) laccase activities were analyzed separately. Laccase activity is expressed as nmol of generated DMOPox per minute per mg of mycelium dry weight.