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. 2021 Nov 26;9:230. doi: 10.1186/s40168-021-01180-0

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — Illustration showing the number and taxonomic distribution of gRNA target sites for metazoa and plants that are available for 18S rRNA sequences in the SILVA SSU database v119 [80]. These gRNA-target-site oligonucleotide sequences are used for designing and synthesizing the taxon-specific and the CRISPR-Cas9-compatible sgRNAs that are used to guide CRISPR-Cas9 to cut the 18S rRNA genes of metazoan or plant hosts, but not those of microeukaryotes (protists and fungi). The node size indicates the number of species at each corresponding taxonomic level, while the size of the edge presents the number of gRNA target sites. Nodes and edges with the highest values are purple, while the smallest ones are gray. Only taxa with more than 50 gRNA target sites per taxon are shown