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Recovery rates of CD9+ uEVs in void urine samples depend on the storage temperature. Freshly prepared, cell-free void urine samples (n = 5) were analyzed by IFCM immediately after antibody staining with anti-CD9 antibodies (no storage) or after storage for 1 month either at room temperature, ((RT) 20 °C), 4 °C, −20 °C, or −80 °C. The recovery of CD9+ objects in stored samples was calculated as the ratio of CD9+ objects before and after storage. ** p ≤ 0.01, **** p ≤ 0.0001.
