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. 2021 Nov 18;22(22):12436. doi: 10.3390/ijms222212436

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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IFCM but not traditional NTA specifically detects EVs for the evaluation of EV preparation methods. Correlation analyses of acquired Western blot band intensities (TSG101 and UMOD) with CD9⁺ object numbers as determined by IFCM or with average particle numbers as determined by NTA (n = 20) applying Spearman’s correlation coefficient analysis. (A) Correlation analysis of CD9⁺ objects and TSG101 band intensities. (B) Correlation analysis of particle numbers and TSG101 band intensities. (C) Correlation analysis of CD9⁺ objects and UMOD band intensities. (D) Correlation analysis of particle numbers and UMOD band intensities. ** p ≤ 0.01, **** p ≤ 0.0001.