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UF-SEC preparation allows the recovery of uEVs with cup-shaped appearance. Representative images of transmission electron microscopic analysis of uEV samples obtained with the different methods as indicated. Size analyses were performed using the ImageJ software. Mean diameters of the EV-like objects were calculated between 67.97 nm (UF-SEC) and 94.73 nm (PEG-UC). Scale bar: 0.2 µm.
