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. 2021 Nov 12;22(22):12273. doi: 10.3390/ijms222212273

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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CSD was unlikely to promote the physical interaction between TRPA1 and SFKs in rat cerebral cortices. (A) Co-immunoprecipitation between TRPA1 and SFKs in untreated rat cerebral cortices. Either TRPA1 or SFKs was immunoprecipitated (IP) followed by immunoblotting (IB) of the pull-down of TRPA1/SFKs with SFKs/TRPA1. FT: flow-through during IP. (B) Co-immunoprecipitation between TRPA1 and SFKs in rat cerebral cortices treated with IgG or anti-TRPA1 antibody (Anti-TRPA1) at 0.8 μg with or without 2 M K⁺-induced CSD. TRPA1 was immunoprecipitated followed by immunoblotting of the pull-down of TRPA1 with SFKs. IgG was used for pull-down in IP as a negative control in (A,B).