Table 1.
Sequences of the three sgRNAs used in this study.
sgRNA Identifier/(Position) * | sgRNA Sequence Including PAM # |
---|---|
sll0574 (15) | GGGGACCAGTTCACCCTTGTCGG |
slr0977 (16) | CCCCCAGAACTGATTATTGAAGCAGGAC |
slr2107 (56) | CCCATGACTGGTTGCGATTGACGAT |
* sgRNA nucleotide binding position counting from the start codon. # underlined nucleotides indicate the protospacer adjacent motif (PAM).