Figure 4.

MCMBP-deficient parasites show defective interpolar spindles, uneven DNA segregation, and aneuploidy without cell cycle arrest. (a) MCMBP^HADD parasites were cultured in the absence of Shld1. Parasites were then prepared for U-ExM, stained with a nuclear stain (SYTOX or SYBR, in cyan), anti-tubulin (in magenta), a membrane stain (BODIPY TRc, in white), and a protein stain (NHS Ester, in grayscale), and visualized using Airyscan microscopy. Arrow indicates DNA staining in bridge-region. Images containing BODIPY TRc are average intensity projections, while those with NHS ester are maximum intensity projections. Slice-by-slice videos of images in 4a are found in Videos S12–S14. Scale bars as labelled in each image, solid bars = XY scale, dashed bar = combined depth of slices used for Z-projection. (b) Schematic representation of phenotypes overserved in Figure 4a.