Figure 4.

Human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) seeded on protein-patterned (Matrigel) hydrogels. (A) Comparison between cells seeded on hydrogels with protein transferred from microscopy coverslips fabricated in a serial process using the method described in [30] and from diced glass chips fabricated in a batch wafer-scale process as described in this paper. Scale bars denote 100 µm. (B,C) Analysis of cells on protein-patterned hydrogels fabricated from diced glass chips. (B) Microscopy images of an alpha-actinin labeled hiPSC-CM seeded on a hydrogel with protein transferred from a diced glass chip. Scale bars denote 25 µm. (C) Two-dimensional scatter plots of the aspect ratio and area and length and width, along with the marginal distributions of the parameters. The results are grouped by hydrogel with debris (objects with area below 200 µm²) filtered out and high aspect ratio cells selected (between 3:1 and 9:1). The criteria for cells that extend well beyond the protein patterns (width above 16 µm) and likely cell doublets (area above 1900 µm²) are indicated by the dashed lines. Cells were fixed for the analysis.