Table 2.
Enzymatic determination for superoxide dismutase, catalase, and glutathione peroxidase.
| Enzyme | Units | Control | DM | Control 150 mg/kg |
DM 150 mg/kg |
Control 300 mg/kg |
DM 300 mg/kg |
Control 600 mg/kg |
DM 600 mg/kg |
|---|---|---|---|---|---|---|---|---|---|
| mSOD | U mg−1 prot | 178.9 ± 17.7 | 321.9 ± 5.9 * | 244.2 ± 5.2 | 313.7 ± 12.4 * | 265.0 ± 14.8 | 303.2 ± 27.0 | 216.5 ± 3.3 | 193.8 ±17.8 |
| Catalase | U mg−1 prot | 31.2 ± 1.6 | 34.7 ± 0.2 | 22.6 ± 0.5 | 21.6 ± 1.0 | 27.5 ± 1.9 | 24.1 ± 1.7 | 36.6 ± 0.9 | 20.1 ± 0.6 * |
| GSH-Px | µM Min−1 Mg−1 prot | 33.4 ± 0.1 | 29.8 ± 0.4 * | 42.5 ± 0.5 | 37.7 ± 1.3 * | 95.2 ± 0.8 | 94.4 ± 1.0 | 98.3 ± 1.6 | 94.5 ± 1.2 |
Values express the mean ± SEM of at least n = 3 rats. ANOVA with post hoc Tukey HSD test for unbalanced data was performed. * p ≤ 0.05 controls versus DM groups. Abbreviations: mSOD, mitochondrial superoxide dismutase; GSH-Px, glutathione peroxidase. Catalase was determined in kidney homogenates, and mSOD and GSH-Px were determined in isolated kidney mitochondria—in both cases, at the end of the 5 weeks of treatments. Source: authors’ own elaboration.