TABLE 1.
Characteristics and nucleotide base sequences of primers and probes used to detect target DNA of HSV
| Gene target | GenBank accession no. (HSV type) | Product size (bp) | Sequencesa |
|---|---|---|---|
| DNA polymerase | M16721 | 215 | GCT CGA GTG CGA AAA AAC GTT C (primer) and CGG GGC GCT CGG CTA AC (primer) |
| 56 | GTA CAT CGG CGT CAT CTG CGG GGG CAA G-FLUOR (probe) and LC-Red 640-T GCT CAT CAA GGG CGT GGA TCT GGT GC-Phos (probe)b | ||
| TK | X03764 (HSV-1) and X01712 (HSV-2) | 335 | GAC MAG CGC CCA GAT AAC AA (primer), MCA GCA TRG CCA GGT CAA GC (primer), AGG CGG TCG ATG TGT CTG TC (HSV-1 capture probe), and AGG CGG TCG GCG TGT TCG GC (HSV-2 capture probe) |
a
Sequences shown are in the 5′ to 3′ direction. The sequence type is indicated in parentheses. Hybridization probes. FLUOR, fluorescein; Phos, phosphate.