Table 2.
Effect of Etpost (2 μg/ml) against the RISK/SAFE kinase inhibitors on functional hemodynamic parameters of the isolated rat heart during the reperfusion time
| % LVDP (mmHg) | % RPP (mmHg).(bpm) | % Heart Rate (bpm) | %Contractility Index (1/s) | % dP/dtmax (mmHg/s) |
% dP/dtmin (mmHg/s) |
Coronary Flow(ml/min) |
|
|---|---|---|---|---|---|---|---|
| IR (Con.)@ | 39.35 ± 15.9 | 35.42 ± 14.3 | 121.8 ± 20.4 | 39.75 ± 16.7 | 38.67 ± 15.7 | 34.49 ± 12.9 | 4.75 ± 0.25 [7] |
| IPOST (Con.) | 79.93 ± 9.52* | 73.30 ± 6.70* | 91.62 ± 5.73* | 67.76 ± 13.5* | 72.91 ± 6.98* | 73.26 ± 6.51* | 7.56 ± 0.05* |
| Etpost (2 μg/ml) | 78.81 ± 3.22* | 67.24 ± 3.76* | 85.62 ± 4.57* | 77.69 ± 3.94* | 75.36 ± 3.61* | 65.85 ± 6.07* | 6.10 ± 0.06* |
| Etpost (2 μg/ml) + Wort | 66.09 ± 12.6#’$ | 67.89 ± 11.2$ | 101.38 ± 15.7#, $ | 72.64 ± 13.6$ | 69.62 ± 12.7$ | 60.19 ± 7.16$ | 3.28 ± 2.42# |
| Etpost (2 μg/ml) + PD | 72.85 ± 12.4$ | 72.69 ± 13.3$ | 98.58 ± 5.03# | 85.78 ± 8.93#, $ | 82.92 ± 15.6#, $ | 66.52 ± 12.7$ | 10.3 ± 1.11# |
| Etpost (2 μg/ml) + AG | 56.37 ± 14.7# | 47.10 ± 19.4# | 70.26 ± 13.9# | 62.13 ± 15.1#, $ | 52.53 ± 15.7# | 46.43 ± 16.9# | 3.05 ± 2.42#, $ |
| Etpost (2 μg/ml) + HD | 74.07 ± 2.86$ | 66.83 ± 2.91$ | 90.73 ± 7.57 | 123.9 ± 5.91#, $ | 76.13 ± 1.85$ | 67.38 ± 1.67$ | 2.84 ± 0.34# |
| IPOST + Wort | 51.73 ± 9.77& | 48.89 ± 9.34& | 73.54 ± 15.92& | 81.72 ± 11.5& | 57.70 ± 8.94& | 49.86 ± 7.95& | 3.83 ± 1.42& |
| IPOST + PD | 43.70 ± 9.97& | 42.06 ± 11.9& | 94.85 ± 16.5 | 40.64 ± 11.0& | 44.97 ± 7.73& | 37.76 ± 5.65& | 5.10 ± 0.77& |
| IPOST + AG | 49.39 ± 13.2& | 46.71 ± 12.2& | 78.87 ± 1.90& | 99.48 ± 26.6& | 54.59 ± 18.7& | 47.38 ± 19.9& | 0.75 ± 0.58& |
| IPOST + HD | 61.62 ± 8.18& | 57.83 ± 9.10& | 90.66 ± 5.43 | 65.33 ± 15.1 | 57.47 ± 13.1& | 56.95 ± 10.0& | 3.00 ± 1.14& |
IR Ischemic reperfusion, IPOST Ischemic postconditioning, dp/dtmax The steepest slope during the upstroke of the pressure, dp/dt min The steepest slope during the downstroke of the pressure, LVDP Left ventricular developed pressure (LV systolic pressure minus LV diastolic pressure), RPP Rate-Pressure Product, Etpost Ethyl acetate fraction of Potentilla reptans root in postconditioning mechanism, Wort Wortmannin (PI3K/Akt inhibitor), PD PD98059 (ERK inhibitor), AG AG490 a JAK/STAT3 inhibitor, HD 5HD a mitoKATP channel blocker. Data are presented as means ± SEM. *P < 0.05 vs. IR; & P < 0.05 vs. IPOST; # P < 0.05 vs. Etpost (2 μg/ml); $ P < 0.05 vs. IPOST+inhibitor. @: [7]