Figure 4.

Tumor incidence rate increases after perturbing tumor suppressors. (A) Intestinal tumors can be observed after perturbing the Notch pathway; notice the cluster of GFP⁺ cells accumulated in the posterior midgut (insert). (B) Quantification of the number of tumor bearing flies after perturbing the BMP, Notch, and JNK pathways. Both Cas9.P2^on/off and u^MCas9^on increase the percentage of tumor bearing flies when gRNAs were used to target Mad, neur, or Notch, while no such effects were seen with Cas9.P2^on. Scale bar: 350 µm, insert—60 µm.