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. 2021 Nov 9;22(22):12127. doi: 10.3390/ijms222212127
PARP1 poly(ADP-ribose)polymerase 1
PAR poly(ADP-ribose)
MD molecular dynamics
FRET Förster resonance energy transfer
spFRET single particle Förster resonance energy transfer
EMSA electrophoretic mobility shift assay
Cy3 and Cy5 fluorescent dyes cyanine 3 and cyanine 5
EPR proximity ratio (FRET efficiency without correction for detection sensitivity and quantum yields of fluorophores)
LN_P, LN_M, LN_D nucleosomes with one 20 bp linker labeled with Cy3/Cy5 at positions 13/91 bp, 35/112 bp or 57/135 bp, respectively
CN_P, CN_M, CN_D core nucleosomes labeled with Cy3/Cy5 at positions 13/91 bp, 35/112 bp or 57/135 bp, respectively