Figure 2.

Grifola frondosa extract (GE) reduces the fat content of C. elegans from L1 to L4 independently of the effect on worm development. (A) Microscopic visualization of the worm fat content of control and GE (10 and 20 µg/mL)-treated worms after staining with Nile Red. (B) Nile Red staining quantification of control and GE-treated (10 and 20 µg/mL) worms. Orlistat (6 µg/mL) was used as the positive control. (C) Oil Red O staining quantification of control and GE-treated worms (20 µg/mL). (D) Length (µM) of GE-treated and untreated worms on day 1 of adulthood. (E) Area (µM²) of GE-treated and untreated worms on day 1 of adulthood. All results are expressed as the mean ± SEM relative to NGM control worms. Significance refers to the effect of the treatments with respect to NGM control worms (ANOVA followed by LSD test, *** p < 0.001). (F) Microscope observation of the presence of eggs (black arrows) and L1 larvae (white arrows) in control (NGM) and GE-supplemented plates.