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. 2021 Nov 13;13(11):802. doi: 10.3390/toxins13110802

Figure 6.

Figure 6

OTA regulates PPARγ by post-translational modification. (a) PPARγ mRNA expression in livers of control and OTA-treated mice (n = 12 in each group). (b) Influence of OTA on the ubiquitination of PPARγ is examined in HEK293T and HepG2 cells. (c) PPARγ expression after OTA and cycloheximide (CHX) treatment. (d) Western blot analysis of SIAH2 expression in livers of control and OTA-treated mice. (e) Endogenous interaction between PPARγ and SIAH2 is examined by immunoprecipitation with SIAH2 antibody in control and OTA-treated HepG2 cells. (f) HepG2 cells are treated with OTA at the concentrations of 5, 10 and 15 μM for 24 h, and endogenous interaction between PPARγ and SIAH2 is examined by immunoprecipitation with SIAH2 antibody. Data shown as the mean ± S.E.M. ns means no significant difference.