Figure 5.

Flow cytometry analysis of immune cells isolated from tumors generated by implantation of 4T1(Luc2) cells premixed with M0 or M1 macrophages. Tumors were homogenized and a single cell suspension was used for immunostaining (see Materials and methods for the details). Flow cytometry was performed to quantify the immune cell populations (%): (a) CD11b⁺; MHCII⁺; CD4⁺; CD8⁺ in total single cells and CD25⁺/FoxP3⁺ in the CD4⁺ population; and (b) double positive CD11b⁺/CD38⁺, CD11b⁺/CD206⁺ cells in total single cells. Representative flow cytometry gating data of CD11b⁺ and CD38⁺ cells are shown. Bars represent the means ± SD (n = 5); * p < 0.05; ** p < 0.01; ns—nonsignificant.