Figure 6.

Inhibition of 4T1 tumor growth by i.t. injection of SFV/IFNg virus. The 4T1 mouse breast tumors were established by subcutaneous (sc.) or orthotopic injections of 2.5 × 10⁵ and 1.25 × 10⁵ 4T1 cells, respectively. Mice received two i.t. injections of SFV vectors (4 × 10⁷ i.u./tumor) or PBS control, as indicated by red arrow time points. The tumor growth curves are shown on the left and the tumor weights of the groups are shown on the right. (a) Treatment of subcutaneous tumors with SFV/IFNg, SFV/Luc, and PBS (control). The next day after virus administration, mice in these groups received i.t. injections of Pam3 solution (10 µg/tumor at day 8 after first virus administration and 15 µg/tumor at day 14 after second virus administration). (b) Treatment of orthotopic tumors with SFV/IFNg, SFV/Luc, and PBS (control). The day after virus administration, mice in these groups similarly received i.t. injections of Pam3 solution (10 µg/tumor at day 5 after first virus administration and 15 µg/tumor at day 11 after second virus administration). (c) Treatment of orthotopic tumors with only Pam3 (or PBS) solution: first Pam3 (10 µg/tumor) i.t. injection at day 5; second Pam3 i.t. injection (15 µg/tumor) at day 11. (d) Treatment of orthotopic tumors only with SFV/IFNg, SFV/Luc, and PBS (control), without the subsequent Pam3 injection. Bars represent the means ± SD (n = 5); * p < 0.05; ** p < 0.01; ns—nonsignificant.