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. 2021 Oct 27;9(11):1247. doi: 10.3390/vaccines9111247

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Flow cytometry of the 4T1 tumors treated with SFV/IFNg, SFV/Luc or PBS (mice were treated as presented in Figure 6d). Tumors were resected, homogenized to obtain a single cell suspension, and the total isolated cells were subjected for immunostaning followed by analysis of forward and side (SSC-A/FSC-A) scattering of cell populations. (a) Schematic representation of the identification of four distinct single-cell populations (representative pictures). (b) Percentage of the respective populations within total single cells (five mice per group). (c) Representative SSC-A/FSC-A data from the PBS, SFV/Luc, and SFV/IFNg groups. The pronounced P2 populations are indicated by arrows. Bars represent the means ± SD (n = 5); ** p < 0.01.